Figure 4From: Exposure to vehicle emissions results in altered blood brain barrier permeability and expression of matrix metalloproteinases and tight junction proteins in mice Reactive oxygen species fluorescence staining of the cerebral cortex and cerebral microvessels from brains of Apo E−/− mice exposed to mixed vehicle emissions or filtered air. Representative dihydroethidium (ethidium, red) fluorescence staining of (frontal) cerebral cortex sections (A,B) and cerebral microvessels (D,E) from brains of Apo E−/− mice exposed to either (A,D) mixed vehicular engine exhaust (MVE, 100 μg PM/m3) or (B,E) filtered air (FA) for 6 hr/day, 30 days. A, B scale bar = 100 μm; D, E scale bar = 10 μm. Arrows indicate areas of increased DHE staining. C, F = graphical quantification of total fluorescence per unit area. n = 5-6 per group, 3 slides (6 section) per animal, 2 sites (areas) each, were used for analysis. *p < 0.050 compared to FA control.Back to article page