Figure 8
Effect of pharmacological inhibitors on the uptake of 50 nm-FITC-SiO 2 and 100 nm-Por-SiO 2 NPs. Cells were pre-treated with inhibitors of main endocytotic pathways: Chlorpromazine (CP) at 25 μM, Monodansylcadaverine (MDC) at 75 μM, EIPA (E) at 75 μM, Amiloride (A) at 1.5 mM, Nystatin (N) at 75 μM and Filipin (F) at 4.5 μM for 30 min and then exposed to A.: 50 nm-FITC-SiO2 NPs at 5 μg/cm2 and inhibitors for 3.5 h. Quantification of the internalization was performed by flow cytometry, after addition of TB. Results are expressed as mean percentage of inhibition of NP uptake in cells not treated with inhibitors ± SD, n = 6–18. B.: 100 nm-Por-SiO2 NPs at 15 μg/cm2 and inhibitors or at 4°C for 3.5 h. Analysis of the internalization was performed by imaging flow cytometry using a mask eroded for 3 μm. Results are expressed as mean Internalization score ± SD of four independent experiments. Data were analyzed by ANOVA, followed by Bonferroni post hoc test. * significantly different from treatment with NPs at 37°C in the absence of inhibitors, p < 0.05.