Figure 6
Internalization of 100 nm-Por-SiO 2 by NCI-H292 cells analyzed by imaging flow cytometry. A. Internalization score obtained using a mask eroded for 3 μm after treatment with 100 nm-Por-SiO2 at 37 °C or 4°C, at different concentrations and at different time points. B. Mean fluorescence intensity (MFI) inside the mask eroded for 3 μm for cells treated with 100 nm-Por-SiO2 at different time points and at different concentrations. Values are expressed as mean IS (A.) or mean value of MFI (B.) ± SD of six independent experiments analyzing at least 500 cells. Data were analyzed by ANOVA, followed by Bonferroni post hoc test. * significantly different from previous time point, p < 0.05. §significantly different from lower concentration, p < 0.05.