Figure 4
Determination of 50 nm-FITC-SiO 2 uptake in NCI-H292 cells by flow cytometry and confocal microscopy. A. 3D reconstruction of a confocal analysis of cells exposed to 50 nm-FITC-SiO2 NPs at 5 μg/cm2 for 4 h at 4°C. Staining of the cells is as follows: Blue - DAPI-stained nuclei, Red – TRITC-phalloidin-stained actin filaments, Green – FITC-labelled SiO2 NPs. Scale bar shows 10 μm. B. The same field of the confocal image shown in the Figure 4A presented as a projection of all images acquired in the stack. C. 3D reconstruction of x,z and y,z-slices of the corresponding regions of the image 4A. The insert shows one selected representative cell and D. Cells were pre-incubated for 30 min at 4°C, and subsequently exposed to 50 nm-FITC-SiO2 at 4°C. Median fluorescence intensity (MFI) of at least 10.000 cells was analysed by FCM without or with 0.11% TB added just before FCM analysis. Results are represented as mean MFI value ± SD, n=3 of one out of 3 independent experiments.