Figure 2
Interaction of 100 nm-Por-SiO 2 NPs with NCI-H292. A. 3D reconstruction of a confocal analysis of cells exposed to 100 nm-Por-SiO2 NPs at 25 μg/cm2 for 24 h. Staining of the cells is as follows: Blue - DAPI-stained nuclei, Green - FITC-phalloidin-stained actin filaments, Red - Porphyrine-labelled SiO2 particles. Scale bar shows 10 μm. B. The same field of the confocal image shown in the Figure 2A presented as a projection of all images acquired in the stack. C. 3D reconstruction of x,z and y,z-slices of the corresponding regions of the image 2A. The insert shows one selected representative cell and D. Cells were exposed to different concentrations of NPs at indicated time points, followed by FCM analysis of median fluorescence intensity (MFI) of at least 10.000 cells. Results are represented as mean MFI value ± SD, n=3 of one out of 3 independent experiments. Data were analyzed by ANOVA, followed by Bonferroni post hoc test. * significantly different from previous time point, p < 0.05.