Figure 10
Colocalization of SiO 2 NPs with proteins specific for different endocytotic vesicles analyzed by confocal microscopy. Cells treated with SiO2 NPs for 4 h were fixed and immunolabelled with Clathrin Heavy Chain antibody (CHC, 8A and 8D), Caveolin-1 antibody (8B and 8E) and Sorting Nexin-5 antibody (SNX-5, 8C and 8F). A, B and C: 50 nm-FITC-SiO2 NPs. Staining is as follows: Blue – DAPI-stained nuclei, Red –CHC, SNX-5, and caveolin-1 labelling, Green – FITC-labelled SiO2 NPs. D, E and F: 100 nm-porphyrine-SiO2 NPs. Staining is as follows: Blue - DAPI-stained nuclei, Green – CHC, SNX-5 and caveolin-1 labelling, Red – Porphyrine-labelled SiO2 NPs. Scale bar shows 10 μm. Pearson’s coefficients were calculated and expressed as mean value ± SD for at least 3 images obtained in three independent experiments.